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KMID : 0381219760080050267
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Journal of RIMSK
1976 Volume.8 No. 5 p.267 ~ p.274
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Experimental studies of using a tissue protein of the epididymis and
seminal vesicle for an antifertility antigen
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Abstract
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Previous studies have shown that rabbit antiserum produced by the tissue protein of rat epididymis and seminal vesicle was capable of immobilizing and agglutinating the sperm of both animals, and the rabbit antiserum against complex antigen of epididymal tissue protein and seminal vesicle tissue protein of rat was most significant on sperm immobilization and agglutination of both animals.
An immunoelectrophoretic classification of the antigens which were used in previous studies has been classified them into more than three major groups in each antigen. None of these components has been studied in great detail. The present study deals with purification and characterization of the fraction which is specific and highly immunogenic from those antigens.
Results are as follows:
1. Specific antigen of the epididymis was isolated including seminal vesicle by the use of series of sephadex G-100 chromatographic purification procedures.
2. Study of the purified fraction by Sephadex G-100 column chromatography,: The antigen of epididymis containing 10.0 mg in 1.0 ml was applied to the 2.5 X40 cm sephadex G-100 column and eluted. An extinction curve was obtained which showed in Fig. 1. with a main peak in tube 22^´25 and a later small peak. The contents of each tube 22^´25 of the main peak and each tube 100~120 of the small peak were analyzed by immunoelectrophoresis. The antigen in tube 24 was found to be a specific antigenic and containing highest protein in these series throughout the four fractions (Fig. 4. CQ. The eluate of the later small peak in tube 100´120 was found to be nonantigenic. The antigen of seminal vesicle was analyzed by the same way and extinction curve was obtained as Fig. 2 with a main peak in tube 20^´28
and a later small peak. The fraction in tube 28 was found to be a specific antigenic and containing highest protein of the seminal vesicle, the later small peak showed nonantigenic.
3. Studies of the purified fraction by immunoelectrophoresis the antigen in tube 24 is defined as the prominent antigenic component of epididymis antigen which forms a distinct long arc and fast diffusion rate in immunoelectrophoresis (Fig. 4. C)
The seminal vesicle antigen in tube 28 forms a distinct long arc and fast diffusion rate in immunoelectrophoresis (Fig. 5. D) which seems to be the prominent antigenic component of seminal vesicle antigen.
4. Approximate molecular weight estimation was performed by Weber and Osborn´s method (Fig. 6). The molecular weight of prominent antigenic component of epididymis was es::mated to be 113, 500 and that of seminal vesicle was 115, 500.
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